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Exploration of twin‐arginine translocation for expression and purification of correctly folded proteins in Escherichia coli
Historically, the general secretory (Sec) pathway of Gram‐negative bacteria has served as the primary route by which heterologous proteins are delivered to the periplasm in numerous expression and engineering applications. Here we have systematically examined the twin‐arginine translocation (Tat) pa...
Autores principales: | Fisher, Adam C., Kim, Jae‐Young, Perez‐Rodriguez, Ritsdeliz, Tullman‐Ercek, Danielle, Fish, Wallace R., Henderson, Lee A., DeLisa, Matthew P. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057487/ https://www.ncbi.nlm.nih.gov/pubmed/21261860 http://dx.doi.org/10.1111/j.1751-7915.2008.00041.x |
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