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A decisive function of transforming growth factor-β/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes

 The mechanism by which transforming growth factor-β (TGFβ) regulates differentiation in human epidermal keratinocytes is still poorly understood. To assess the role of Smad signaling, we engineered human HaCaT keratinocytes either expressing small interfering RNA against Smads2, 3, and 4 or overexp...

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Autores principales: Buschke, Susanne, Stark, Hans-Jürgen, Cerezo, Ana, Prätzel-Wunder, Silke, Boehnke, Karsten, Kollar, Jasmin, Langbein, Lutz, Heldin, Carl-Henrik, Boukamp, Petra
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057703/
https://www.ncbi.nlm.nih.gov/pubmed/21289094
http://dx.doi.org/10.1091/mbc.E10-11-0879
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author Buschke, Susanne
Stark, Hans-Jürgen
Cerezo, Ana
Prätzel-Wunder, Silke
Boehnke, Karsten
Kollar, Jasmin
Langbein, Lutz
Heldin, Carl-Henrik
Boukamp, Petra
author_facet Buschke, Susanne
Stark, Hans-Jürgen
Cerezo, Ana
Prätzel-Wunder, Silke
Boehnke, Karsten
Kollar, Jasmin
Langbein, Lutz
Heldin, Carl-Henrik
Boukamp, Petra
author_sort Buschke, Susanne
collection PubMed
description  The mechanism by which transforming growth factor-β (TGFβ) regulates differentiation in human epidermal keratinocytes is still poorly understood. To assess the role of Smad signaling, we engineered human HaCaT keratinocytes either expressing small interfering RNA against Smads2, 3, and 4 or overexpressing Smad7 and verified impaired Smad signaling as decreased Smad phosphorylation, aberrant nuclear translocation, and altered target gene expression. Besides abrogation of TGFβ-dependent growth inhibition in conventional cultures, epidermal morphogenesis and differentiation in organotypic cultures were disturbed, resulting in altered tissue homeostasis with suprabasal proliferation and hyperplasia upon TGFβ treatment. Neutralizing antibodies against TGFβ, similar to blocking the actions of EGF-receptor or keratinocyte growth factor, caused significant growth reduction of Smad7-overexpressing cells, thereby demonstrating that epithelial hyperplasia was attributed to TGFβ-induced “dermis”-derived growth promoting factors. Furthermore impaired Smad signaling not only blocked the epidermal differentiation process or caused epidermal-to-mesenchymal transition but induced a switch to a complex alternative differentiation program, best characterized as mucous/intestinal-type epithelial differentiation. As the same alternative phenotype evolved from both modes of Smad-pathway interference, and reduction of Smad7-overexpression caused reversion to epidermal differentiation, our data suggest that functional TGFβ/Smad signaling, besides regulating epidermal tissue homeostasis, is not only essential for terminal epidermal differentiation but crucial in programming different epithelial differentiation routes.
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spelling pubmed-30577032011-05-30 A decisive function of transforming growth factor-β/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes Buschke, Susanne Stark, Hans-Jürgen Cerezo, Ana Prätzel-Wunder, Silke Boehnke, Karsten Kollar, Jasmin Langbein, Lutz Heldin, Carl-Henrik Boukamp, Petra Mol Biol Cell Articles  The mechanism by which transforming growth factor-β (TGFβ) regulates differentiation in human epidermal keratinocytes is still poorly understood. To assess the role of Smad signaling, we engineered human HaCaT keratinocytes either expressing small interfering RNA against Smads2, 3, and 4 or overexpressing Smad7 and verified impaired Smad signaling as decreased Smad phosphorylation, aberrant nuclear translocation, and altered target gene expression. Besides abrogation of TGFβ-dependent growth inhibition in conventional cultures, epidermal morphogenesis and differentiation in organotypic cultures were disturbed, resulting in altered tissue homeostasis with suprabasal proliferation and hyperplasia upon TGFβ treatment. Neutralizing antibodies against TGFβ, similar to blocking the actions of EGF-receptor or keratinocyte growth factor, caused significant growth reduction of Smad7-overexpressing cells, thereby demonstrating that epithelial hyperplasia was attributed to TGFβ-induced “dermis”-derived growth promoting factors. Furthermore impaired Smad signaling not only blocked the epidermal differentiation process or caused epidermal-to-mesenchymal transition but induced a switch to a complex alternative differentiation program, best characterized as mucous/intestinal-type epithelial differentiation. As the same alternative phenotype evolved from both modes of Smad-pathway interference, and reduction of Smad7-overexpression caused reversion to epidermal differentiation, our data suggest that functional TGFβ/Smad signaling, besides regulating epidermal tissue homeostasis, is not only essential for terminal epidermal differentiation but crucial in programming different epithelial differentiation routes. The American Society for Cell Biology 2011-03-15 /pmc/articles/PMC3057703/ /pubmed/21289094 http://dx.doi.org/10.1091/mbc.E10-11-0879 Text en © 2011 Buschke et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Buschke, Susanne
Stark, Hans-Jürgen
Cerezo, Ana
Prätzel-Wunder, Silke
Boehnke, Karsten
Kollar, Jasmin
Langbein, Lutz
Heldin, Carl-Henrik
Boukamp, Petra
A decisive function of transforming growth factor-β/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes
title A decisive function of transforming growth factor-β/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes
title_full A decisive function of transforming growth factor-β/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes
title_fullStr A decisive function of transforming growth factor-β/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes
title_full_unstemmed A decisive function of transforming growth factor-β/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes
title_short A decisive function of transforming growth factor-β/Smad signaling in tissue morphogenesis and differentiation of human HaCaT keratinocytes
title_sort decisive function of transforming growth factor-β/smad signaling in tissue morphogenesis and differentiation of human hacat keratinocytes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3057703/
https://www.ncbi.nlm.nih.gov/pubmed/21289094
http://dx.doi.org/10.1091/mbc.E10-11-0879
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