Robust RT-qPCR Data Normalization: Validation and Selection of Internal Reference Genes during Post-Experimental Data Analysis

Reverse transcription and real-time PCR (RT-qPCR) has been widely used for rapid quantification of relative gene expression. To offset technical confounding variations, stably-expressed internal reference genes are measured simultaneously along with target genes for data normalization. Statistic met...

Descripción completa

Detalles Bibliográficos
Autores principales: Ling, Daijun, Salvaterra, Paul M.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058000/
https://www.ncbi.nlm.nih.gov/pubmed/21423626
http://dx.doi.org/10.1371/journal.pone.0017762

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3058000/
https://www.ncbi.nlm.nih.gov/pubmed/21423626
http://dx.doi.org/10.1371/journal.pone.0017762

Ejemplares similares