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Cultivation of E. coli carrying a plasmid-based Measles vaccine construct (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques

BACKGROUND: Plasmid-based measles vaccines offer great promises over the conventional fertilised egg method such as ease of manufacture and mimic wild-type intracellular antigen expression. The increasing number of clinical trials on plasmid-based measles vaccines has triggered the need to make more...

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Detalles Bibliográficos
Autores principales: Ongkudon, Clarence M, Pickering, Raelene, Webster, Diane, Danquah, Michael K
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059270/
https://www.ncbi.nlm.nih.gov/pubmed/21375765
http://dx.doi.org/10.1186/1475-2859-10-16