Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression

BACKGROUND: Less than 30% of malignant gliomas respond to adjuvant chemotherapy. Here, we have asked whether variations in the constitutive expression of early-growth response factor 1 (EGR-1) predicted acute cytotoxicity and clonogenic cell death in vitro, induced by six different chemotherapics. M...

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Autores principales: Calogero, Antonella, Porcellini, Antonio, Lombari, Vincenza, Fabbiano, Cinzia, Arcella, Antonietta, Miscusi, Massimo, Ponti, Donatella, Ragona, Giuseppe
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Primary Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059282/
https://www.ncbi.nlm.nih.gov/pubmed/21366897
http://dx.doi.org/10.1186/1475-2867-11-5
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author Calogero, Antonella
Porcellini, Antonio
Lombari, Vincenza
Fabbiano, Cinzia
Arcella, Antonietta
Miscusi, Massimo
Ponti, Donatella
Ragona, Giuseppe
author_facet Calogero, Antonella
Porcellini, Antonio
Lombari, Vincenza
Fabbiano, Cinzia
Arcella, Antonietta
Miscusi, Massimo
Ponti, Donatella
Ragona, Giuseppe
author_sort Calogero, Antonella
collection PubMed
description BACKGROUND: Less than 30% of malignant gliomas respond to adjuvant chemotherapy. Here, we have asked whether variations in the constitutive expression of early-growth response factor 1 (EGR-1) predicted acute cytotoxicity and clonogenic cell death in vitro, induced by six different chemotherapics. MATERIALS AND METHODS: Cytotoxicity assays were performed on cells derived from fresh tumor explants of 18 human cases of malignant glioma. In addition to EGR-1, tumor cultures were investigated for genetic alterations and the expression of cancer regulating factors, related to the p53 pathway. RESULTS: We found that sensitivity to cisplatin correlates significantly with levels of EGR-1 expression in tumors with wild-type p53/INK4a/p16 status. CONCLUSION: Increased knowledge of the mechanisms regulating EGR-1 expression in wild-type p53/INK4a/p16 cases of glioma may help in the design of new chemotherapeutic strategies for these tumors.
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spelling pubmed-30592822011-03-17 Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression Calogero, Antonella Porcellini, Antonio Lombari, Vincenza Fabbiano, Cinzia Arcella, Antonietta Miscusi, Massimo Ponti, Donatella Ragona, Giuseppe Cancer Cell Int Primary Research BACKGROUND: Less than 30% of malignant gliomas respond to adjuvant chemotherapy. Here, we have asked whether variations in the constitutive expression of early-growth response factor 1 (EGR-1) predicted acute cytotoxicity and clonogenic cell death in vitro, induced by six different chemotherapics. MATERIALS AND METHODS: Cytotoxicity assays were performed on cells derived from fresh tumor explants of 18 human cases of malignant glioma. In addition to EGR-1, tumor cultures were investigated for genetic alterations and the expression of cancer regulating factors, related to the p53 pathway. RESULTS: We found that sensitivity to cisplatin correlates significantly with levels of EGR-1 expression in tumors with wild-type p53/INK4a/p16 status. CONCLUSION: Increased knowledge of the mechanisms regulating EGR-1 expression in wild-type p53/INK4a/p16 cases of glioma may help in the design of new chemotherapeutic strategies for these tumors. BioMed Central 2011-03-02 /pmc/articles/PMC3059282/ /pubmed/21366897 http://dx.doi.org/10.1186/1475-2867-11-5 Text en Copyright ©2011 Calogero et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Primary Research
Calogero, Antonella
Porcellini, Antonio
Lombari, Vincenza
Fabbiano, Cinzia
Arcella, Antonietta
Miscusi, Massimo
Ponti, Donatella
Ragona, Giuseppe
Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression
title Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression
title_full Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression
title_fullStr Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression
title_full_unstemmed Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression
title_short Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression
title_sort sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of egr-1 expression
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059282/
https://www.ncbi.nlm.nih.gov/pubmed/21366897
http://dx.doi.org/10.1186/1475-2867-11-5
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