Histidyl-tRNA Synthetase Urzymes: CLASS I AND II AMINOACYL tRNA SYNTHETASE URZYMES HAVE COMPARABLE CATALYTIC ACTIVITIES FOR COGNATE AMINO ACID ACTIVATION

Four minimal (119 - 145 residue) active site fragments of Escherichia coli Class II histidyl-tRNA synthetase were constructed, expressed as maltose-binding protein fusions, and assayed for histidine activation as fusion proteins and after TEV cleavage, using the (32)PP(i) exchange assay. All contain...

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Detalles Bibliográficos
Autores principales: Li, Li, Weinreb, Violetta, Francklyn, Christopher, Carter, Charles W.
Formato: Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2011
Materias:
Enzymology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060492/
https://www.ncbi.nlm.nih.gov/pubmed/21270472
http://dx.doi.org/10.1074/jbc.M110.198929

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060492/
https://www.ncbi.nlm.nih.gov/pubmed/21270472
http://dx.doi.org/10.1074/jbc.M110.198929

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