Engineered split in Pfu DNA polymerase fingers domain improves incorporation of nucleotide γ-phosphate derivative

Using compartmentalized self-replication (CSR), we evolved a version of Pyrococcus furiosus (Pfu) DNA polymerase that tolerates modification of the γ-phosphate of an incoming nucleotide. A Q484R mutation in α-helix P of the fingers domain, coupled with an unintended translational termination-reiniti...

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Detalles Bibliográficos
Autores principales: Hansen, Connie J., Wu, Lydia, Fox, Jeffrey D., Arezi, Bahram, Hogrefe, Holly H.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061061/
https://www.ncbi.nlm.nih.gov/pubmed/21062827
http://dx.doi.org/10.1093/nar/gkq1053

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061061/
https://www.ncbi.nlm.nih.gov/pubmed/21062827
http://dx.doi.org/10.1093/nar/gkq1053

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