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Engineered split in Pfu DNA polymerase fingers domain improves incorporation of nucleotide γ-phosphate derivative
Using compartmentalized self-replication (CSR), we evolved a version of Pyrococcus furiosus (Pfu) DNA polymerase that tolerates modification of the γ-phosphate of an incoming nucleotide. A Q484R mutation in α-helix P of the fingers domain, coupled with an unintended translational termination-reiniti...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061061/ https://www.ncbi.nlm.nih.gov/pubmed/21062827 http://dx.doi.org/10.1093/nar/gkq1053 |