Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling

This work identifies the combination of enzymatic transfer and click labeling as an efficient method for the site-specific tagging of RNA molecules for biophysical studies. A double-activated analog of the ubiquitous co-substrate S-adenosyl-l-methionine was employed to enzymatically transfer a five...

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Detalles Bibliográficos
Autores principales: Motorin, Yuri, Burhenne, Jürgen, Teimer, Roman, Koynov, Kaloian, Willnow, Sophie, Weinhold, Elmar, Helm, Mark
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Synthetic Biology and Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061074/
https://www.ncbi.nlm.nih.gov/pubmed/21037259
http://dx.doi.org/10.1093/nar/gkq825

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061074/
https://www.ncbi.nlm.nih.gov/pubmed/21037259
http://dx.doi.org/10.1093/nar/gkq825

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