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Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling
This work identifies the combination of enzymatic transfer and click labeling as an efficient method for the site-specific tagging of RNA molecules for biophysical studies. A double-activated analog of the ubiquitous co-substrate S-adenosyl-l-methionine was employed to enzymatically transfer a five...
| Autores principales: | , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061074/ https://www.ncbi.nlm.nih.gov/pubmed/21037259 http://dx.doi.org/10.1093/nar/gkq825 |