Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase

Using a component of the Escherichia coli protein degradation machinery, we have established a system to regulate protein stability in mycobacteria. A protein tag derived from the E. coli SsrA degradation signal did not affect several reporter proteins in wild-type Mycobacterium smegmatis or Mycobac...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Jee-Hyun, Wei, Jun-Rong, Wallach, Joshua B., Robbins, Rebekkah S., Rubin, Eric J., Schnappinger, Dirk
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064785/
https://www.ncbi.nlm.nih.gov/pubmed/21075796
http://dx.doi.org/10.1093/nar/gkq1149

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064785/
https://www.ncbi.nlm.nih.gov/pubmed/21075796
http://dx.doi.org/10.1093/nar/gkq1149

Ejemplares similares