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Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase
Using a component of the Escherichia coli protein degradation machinery, we have established a system to regulate protein stability in mycobacteria. A protein tag derived from the E. coli SsrA degradation signal did not affect several reporter proteins in wild-type Mycobacterium smegmatis or Mycobac...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064785/ https://www.ncbi.nlm.nih.gov/pubmed/21075796 http://dx.doi.org/10.1093/nar/gkq1149 |