Improved nucleotide selectivity and termination of 3′-OH unblocked reversible terminators by molecular tuning of 2-nitrobenzyl alkylated HOMedU triphosphates

We describe a novel 3′-OH unblocked reversible terminator with the potential to improve accuracy and read-lengths in next-generation sequencing (NGS) technologies. This terminator is based on 5-hydroxymethyl-2′-deoxyuridine triphosphate (HOMedUTP), a hypermodified nucleotide found naturally in the g...

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Detalles Bibliográficos
Autores principales: Litosh, Vladislav A., Wu, Weidong, Stupi, Brian P., Wang, Jinchun, Morris, Sidney E., Hersh, Megan N., Metzker, Michael L.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064798/
https://www.ncbi.nlm.nih.gov/pubmed/21227920
http://dx.doi.org/10.1093/nar/gkq1293

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064798/
https://www.ncbi.nlm.nih.gov/pubmed/21227920
http://dx.doi.org/10.1093/nar/gkq1293

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