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Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification
Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products c...
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Formato: | Texto |
Lenguaje: | English |
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SAGE-Hindawi Access to Research
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065168/ https://www.ncbi.nlm.nih.gov/pubmed/21527985 http://dx.doi.org/10.4061/2011/838232 |
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author | Burrell, A. Foy, C. Burns, M. |
author_facet | Burrell, A. Foy, C. Burns, M. |
author_sort | Burrell, A. |
collection | PubMed |
description | Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients. |
format | Text |
id | pubmed-3065168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SAGE-Hindawi Access to Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-30651682011-04-28 Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification Burrell, A. Foy, C. Burns, M. Biotechnol Res Int Research Article Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients. SAGE-Hindawi Access to Research 2011-03-06 /pmc/articles/PMC3065168/ /pubmed/21527985 http://dx.doi.org/10.4061/2011/838232 Text en Copyright © 2011 A. Burrell et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Burrell, A. Foy, C. Burns, M. Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification |
title | Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification |
title_full | Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification |
title_fullStr | Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification |
title_full_unstemmed | Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification |
title_short | Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification |
title_sort | applicability of three alternative instruments for food authenticity analysis: gmo identification |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065168/ https://www.ncbi.nlm.nih.gov/pubmed/21527985 http://dx.doi.org/10.4061/2011/838232 |
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