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Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification

Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products c...

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Detalles Bibliográficos
Autores principales: Burrell, A., Foy, C., Burns, M.
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065168/
https://www.ncbi.nlm.nih.gov/pubmed/21527985
http://dx.doi.org/10.4061/2011/838232
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author Burrell, A.
Foy, C.
Burns, M.
author_facet Burrell, A.
Foy, C.
Burns, M.
author_sort Burrell, A.
collection PubMed
description Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients.
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spelling pubmed-30651682011-04-28 Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification Burrell, A. Foy, C. Burns, M. Biotechnol Res Int Research Article Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients. SAGE-Hindawi Access to Research 2011-03-06 /pmc/articles/PMC3065168/ /pubmed/21527985 http://dx.doi.org/10.4061/2011/838232 Text en Copyright © 2011 A. Burrell et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Burrell, A.
Foy, C.
Burns, M.
Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification
title Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification
title_full Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification
title_fullStr Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification
title_full_unstemmed Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification
title_short Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification
title_sort applicability of three alternative instruments for food authenticity analysis: gmo identification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065168/
https://www.ncbi.nlm.nih.gov/pubmed/21527985
http://dx.doi.org/10.4061/2011/838232
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