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Impact of Quenching Failure of Cy Dyes in Differential Gel Electrophoresis

BACKGROUND: Differential gel electrophoresis (DIGE) is a technology widely used for protein expression analysis. It is based on labelling with fluorescent Cy dyes. In comparative fluorescence gel electrophoresis experiments, however, unspecific labelling using N-hydroxy-succinimide-ester-based label...

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Detalles Bibliográficos
Autores principales: Wang, Weiqun, Ackermann, Doreen, Mehlich, Anna-Maria, König, Simone
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068157/
https://www.ncbi.nlm.nih.gov/pubmed/21479167
http://dx.doi.org/10.1371/journal.pone.0018098