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The Use of a Stringent Selection System Allows the Identification of DNA Elements that Augment Gene Expression

The use of high stringency selection systems often results in the induction of very few recombinant mammalian cell lines, which limits the ability to isolate a cell line with favorable characteristics. The employment of for instance STAR elements in DNA constructs elevates the induced number of colo...

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Detalles Bibliográficos
Autores principales: Hoeksema, Femke, van Blokland, Rik, Siep, Michel, Hamer, Karien, Siersma, Tjalling, den Blaauwen, Jan, Verhees, John, Otte, Arie P.
Formato: Texto
Lenguaje:English
Publicado: Humana Press Inc 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068247/
https://www.ncbi.nlm.nih.gov/pubmed/21046488
http://dx.doi.org/10.1007/s12033-010-9344-8