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The Use of a Stringent Selection System Allows the Identification of DNA Elements that Augment Gene Expression
The use of high stringency selection systems often results in the induction of very few recombinant mammalian cell lines, which limits the ability to isolate a cell line with favorable characteristics. The employment of for instance STAR elements in DNA constructs elevates the induced number of colo...
Autores principales: | Hoeksema, Femke, van Blokland, Rik, Siep, Michel, Hamer, Karien, Siersma, Tjalling, den Blaauwen, Jan, Verhees, John, Otte, Arie P. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Humana Press Inc
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068247/ https://www.ncbi.nlm.nih.gov/pubmed/21046488 http://dx.doi.org/10.1007/s12033-010-9344-8 |
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