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Identification of a Cryptic Prokaryotic Promoter within the cDNA Encoding the 5′ End of Dengue Virus RNA Genome

Infectious cDNA clones of RNA viruses are important research tools, but flavivirus cDNA clones have proven difficult to assemble and propagate in bacteria. This has been attributed to genetic instability and/or host cell toxicity, however the mechanism leading to these difficulties has not been full...

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Detalles Bibliográficos
Autores principales: Li, Dongsheng, Aaskov, John, Lott, William B.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3069047/
https://www.ncbi.nlm.nih.gov/pubmed/21483867
http://dx.doi.org/10.1371/journal.pone.0018197