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CTLA4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation
BACKGROUND: Dendritic cells (DCs) initiate immune responses through their direct interaction with effector cells. However, the mechanism by which DC activity is regulated is not well defined. Previous studies have shown that CTLA4 on T cells regulates DCs function by "cross-talk". We inves...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070687/ https://www.ncbi.nlm.nih.gov/pubmed/21414236 http://dx.doi.org/10.1186/1471-2172-12-21 |
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author | Wang, Xiong B Fan, Zhong Z Anton, Doina Vollenhoven, Annika V Ni, Zhen H Chen, Xiao F Lefvert, Ann K |
author_facet | Wang, Xiong B Fan, Zhong Z Anton, Doina Vollenhoven, Annika V Ni, Zhen H Chen, Xiao F Lefvert, Ann K |
author_sort | Wang, Xiong B |
collection | PubMed |
description | BACKGROUND: Dendritic cells (DCs) initiate immune responses through their direct interaction with effector cells. However, the mechanism by which DC activity is regulated is not well defined. Previous studies have shown that CTLA4 on T cells regulates DCs function by "cross-talk". We investigated whether there is an intrinsic regulatory mechanism in DCs, with CTLA4 as a candidate regulator. RESULTS: We confirmed via RT-PCR and flow cytometry the natural expression of CTLA4 on mature DCs derived from human monocytes. Approximately 8% CD1a-positive cells express CTLA4 both on surface and intracellular, whereas 10% CD1a-negative cells express CTLA4 intracellularly, but little expression was observed on the cell surface. The cross-linking of CTLA4 inhibits DCs maturation and antigen presentation in vitro, but does not inhibit endocytosis. CONCLUSIONS: CTLA4 is expressed by DCs and plays an inhibitory role. CTLA4-expressing DCs may represent a group of regulatory DCs. Because of its wide distribution on different cell types, CTLA4 may play a general role in regulating immune responses. |
format | Text |
id | pubmed-3070687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30706872011-04-05 CTLA4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation Wang, Xiong B Fan, Zhong Z Anton, Doina Vollenhoven, Annika V Ni, Zhen H Chen, Xiao F Lefvert, Ann K BMC Immunol Research Article BACKGROUND: Dendritic cells (DCs) initiate immune responses through their direct interaction with effector cells. However, the mechanism by which DC activity is regulated is not well defined. Previous studies have shown that CTLA4 on T cells regulates DCs function by "cross-talk". We investigated whether there is an intrinsic regulatory mechanism in DCs, with CTLA4 as a candidate regulator. RESULTS: We confirmed via RT-PCR and flow cytometry the natural expression of CTLA4 on mature DCs derived from human monocytes. Approximately 8% CD1a-positive cells express CTLA4 both on surface and intracellular, whereas 10% CD1a-negative cells express CTLA4 intracellularly, but little expression was observed on the cell surface. The cross-linking of CTLA4 inhibits DCs maturation and antigen presentation in vitro, but does not inhibit endocytosis. CONCLUSIONS: CTLA4 is expressed by DCs and plays an inhibitory role. CTLA4-expressing DCs may represent a group of regulatory DCs. Because of its wide distribution on different cell types, CTLA4 may play a general role in regulating immune responses. BioMed Central 2011-03-18 /pmc/articles/PMC3070687/ /pubmed/21414236 http://dx.doi.org/10.1186/1471-2172-12-21 Text en Copyright ©2011 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Xiong B Fan, Zhong Z Anton, Doina Vollenhoven, Annika V Ni, Zhen H Chen, Xiao F Lefvert, Ann K CTLA4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation |
title | CTLA4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation |
title_full | CTLA4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation |
title_fullStr | CTLA4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation |
title_full_unstemmed | CTLA4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation |
title_short | CTLA4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation |
title_sort | ctla4 is expressed on mature dendritic cells derived from human monocytes and influences their maturation and antigen presentation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070687/ https://www.ncbi.nlm.nih.gov/pubmed/21414236 http://dx.doi.org/10.1186/1471-2172-12-21 |
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