Cargando…
Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing
We combined rapid microfluidic mixing with single-molecule Förster Resonance Energy Transfer to study the folding kinetics of the intrinsically disordered human protein α-synuclein. The time-resolution of 0.2 ms revealed initial collapse of the unfolded protein induced by binding with lipid-mimics a...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
2011
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071799/ https://www.ncbi.nlm.nih.gov/pubmed/21297620 http://dx.doi.org/10.1038/nmeth.1568 |