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Protein-Protein Fusion Catalyzed by Sortase A

Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligati...

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Detalles Bibliográficos
Autores principales: Levary, David A., Parthasarathy, Ranganath, Boder, Eric T., Ackerman, Margaret E.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071835/
https://www.ncbi.nlm.nih.gov/pubmed/21494692
http://dx.doi.org/10.1371/journal.pone.0018342