Protein-Protein Fusion Catalyzed by Sortase A

Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligati...

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Detalles Bibliográficos
Autores principales: Levary, David A., Parthasarathy, Ranganath, Boder, Eric T., Ackerman, Margaret E.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071835/
https://www.ncbi.nlm.nih.gov/pubmed/21494692
http://dx.doi.org/10.1371/journal.pone.0018342
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author Levary, David A.
Parthasarathy, Ranganath
Boder, Eric T.
Ackerman, Margaret E.
author_facet Levary, David A.
Parthasarathy, Ranganath
Boder, Eric T.
Ackerman, Margaret E.
author_sort Levary, David A.
collection PubMed
description Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of protein domains with preserved functionality — demonstrating the robust and facile nature of this reaction.
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spelling pubmed-30718352011-04-14 Protein-Protein Fusion Catalyzed by Sortase A Levary, David A. Parthasarathy, Ranganath Boder, Eric T. Ackerman, Margaret E. PLoS One Research Article Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of protein domains with preserved functionality — demonstrating the robust and facile nature of this reaction. Public Library of Science 2011-04-06 /pmc/articles/PMC3071835/ /pubmed/21494692 http://dx.doi.org/10.1371/journal.pone.0018342 Text en Levary et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Levary, David A.
Parthasarathy, Ranganath
Boder, Eric T.
Ackerman, Margaret E.
Protein-Protein Fusion Catalyzed by Sortase A
title Protein-Protein Fusion Catalyzed by Sortase A
title_full Protein-Protein Fusion Catalyzed by Sortase A
title_fullStr Protein-Protein Fusion Catalyzed by Sortase A
title_full_unstemmed Protein-Protein Fusion Catalyzed by Sortase A
title_short Protein-Protein Fusion Catalyzed by Sortase A
title_sort protein-protein fusion catalyzed by sortase a
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071835/
https://www.ncbi.nlm.nih.gov/pubmed/21494692
http://dx.doi.org/10.1371/journal.pone.0018342
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