Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

BACKGROUND: Pichia pastoris is a widely-used host for recombinant protein production. Initial screening for both suitable clones and optimum culture conditions is typically carried out in multi-well plates. This is followed by up-scaling either to shake-flasks or continuously stirred tank bioreactor...

Descripción completa

Detalles Bibliográficos
Autores principales: Routledge, Sarah J, Hewitt, Christopher J, Bora, Nagamani, Bill, Roslyn M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072307/
https://www.ncbi.nlm.nih.gov/pubmed/21426555
http://dx.doi.org/10.1186/1475-2859-10-17

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072307/
https://www.ncbi.nlm.nih.gov/pubmed/21426555
http://dx.doi.org/10.1186/1475-2859-10-17

Ejemplares similares