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Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield
BACKGROUND: Pichia pastoris is a widely-used host for recombinant protein production. Initial screening for both suitable clones and optimum culture conditions is typically carried out in multi-well plates. This is followed by up-scaling either to shake-flasks or continuously stirred tank bioreactor...
Autores principales: | Routledge, Sarah J, Hewitt, Christopher J, Bora, Nagamani, Bill, Roslyn M |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072307/ https://www.ncbi.nlm.nih.gov/pubmed/21426555 http://dx.doi.org/10.1186/1475-2859-10-17 |
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