Confined Activation and Subdiffractive Localization Enables Whole-Cell PALM with Genetically Expressed Probes

We demonstrate 3D superresolution microscopy in whole fixed cells using photoactivated localization microscopy (PALM). The use of the bright, genetically expressed fluorescent marker photoactivatable mCherry (PA-mCherry1) in combination with near diffraction-limited confinement of photoactivation us...

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Detalles Bibliográficos
Autores principales: York, Andrew G., Ghitani, Alireza, Vaziri, Alipasha, Davidson, Michael W., Shroff, Hari
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3073501/
https://www.ncbi.nlm.nih.gov/pubmed/21317909
http://dx.doi.org/10.1038/nmeth.1571

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3073501/
https://www.ncbi.nlm.nih.gov/pubmed/21317909
http://dx.doi.org/10.1038/nmeth.1571

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