Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔC expression strain

BACKGROUND: The non-pathogenic bacterium Mycobacterium smegmatis is widely used as a near-native expression host for the purification of Mycobacterium tuberculosis proteins. Unfortunately, the Hsp60 chaperone GroEL1, which is relatively highly expressed, is often co-purified with polyhistidine-tagge...

Descripción completa

Detalles Bibliográficos
Autores principales: Noens, Elke E, Williams, Chris, Anandhakrishnan, Madhankumar, Poulsen, Christian, Ehebauer, Matthias T, Wilmanns, Matthias
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076238/
https://www.ncbi.nlm.nih.gov/pubmed/21439037
http://dx.doi.org/10.1186/1472-6750-11-27

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076238/
https://www.ncbi.nlm.nih.gov/pubmed/21439037
http://dx.doi.org/10.1186/1472-6750-11-27

Ejemplares similares