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Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔC expression strain
BACKGROUND: The non-pathogenic bacterium Mycobacterium smegmatis is widely used as a near-native expression host for the purification of Mycobacterium tuberculosis proteins. Unfortunately, the Hsp60 chaperone GroEL1, which is relatively highly expressed, is often co-purified with polyhistidine-tagge...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076238/ https://www.ncbi.nlm.nih.gov/pubmed/21439037 http://dx.doi.org/10.1186/1472-6750-11-27 |