Local De Novo Assembly of RAD Paired-End Contigs Using Short Sequencing Reads

Despite the power of massively parallel sequencing platforms, a drawback is the short length of the sequence reads produced. We demonstrate that short reads can be locally assembled into longer contigs using paired-end sequencing of restriction-site associated DNA (RAD-PE) fragments. We use this RAD...

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Detalles Bibliográficos
Autores principales: Etter, Paul D., Preston, Jessica L., Bassham, Susan, Cresko, William A., Johnson, Eric A.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076424/
https://www.ncbi.nlm.nih.gov/pubmed/21541009
http://dx.doi.org/10.1371/journal.pone.0018561

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076424/
https://www.ncbi.nlm.nih.gov/pubmed/21541009
http://dx.doi.org/10.1371/journal.pone.0018561

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