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Local De Novo Assembly of RAD Paired-End Contigs Using Short Sequencing Reads
Despite the power of massively parallel sequencing platforms, a drawback is the short length of the sequence reads produced. We demonstrate that short reads can be locally assembled into longer contigs using paired-end sequencing of restriction-site associated DNA (RAD-PE) fragments. We use this RAD...
Autores principales: | Etter, Paul D., Preston, Jessica L., Bassham, Susan, Cresko, William A., Johnson, Eric A. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3076424/ https://www.ncbi.nlm.nih.gov/pubmed/21541009 http://dx.doi.org/10.1371/journal.pone.0018561 |
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