Genetic selection designed to stabilize proteins uncovers a chaperone called Spy

To optimize the in vivo folding of proteins, we linked protein stability to antibiotic resistance, thereby forcing bacteria to effectively fold and stabilize proteins. When we challenged Escherichia coli to stabilize a very unstable periplasmic protein, it massively overproduced a periplasmic protei...

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Detalles Bibliográficos
Autores principales: Quan, Shu, Koldewey, Philipp, Tapley, Tim, Kirsch, Nadine, Ruane, Karen M., Pfizenmaier, Jennifer, Shi, Rong, Hofmann, Stephan, Foit, Linda, Ren, Guoping, Jakob, Ursula, Xu, Zhaohui, Cygler, Miroslaw, Bardwell, James C. A.
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079333/
https://www.ncbi.nlm.nih.gov/pubmed/21317898
http://dx.doi.org/10.1038/nsmb.2016

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079333/
https://www.ncbi.nlm.nih.gov/pubmed/21317898
http://dx.doi.org/10.1038/nsmb.2016

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