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Genetic selection designed to stabilize proteins uncovers a chaperone called Spy
To optimize the in vivo folding of proteins, we linked protein stability to antibiotic resistance, thereby forcing bacteria to effectively fold and stabilize proteins. When we challenged Escherichia coli to stabilize a very unstable periplasmic protein, it massively overproduced a periplasmic protei...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079333/ https://www.ncbi.nlm.nih.gov/pubmed/21317898 http://dx.doi.org/10.1038/nsmb.2016 |