Quantitative real-time PCR with SYBR Green detection to assess gene duplication in insects: study of gene dosage in Drosophila melanogaster (Diptera) and in Ostrinia nubilalis (Lepidoptera)

BACKGROUND: The accurate determination of the number of copies of a gene in the genome (gene dosage) is essential for a number of genetic analyses. Quantitative real time PCR (qPCR) with TaqMan detection has shown advantages over traditional Southern-blot and FISH techniques, however the high costs...

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Detalles Bibliográficos
Autores principales: Bel, Yolanda, Ferré, Juan, Escriche, Baltasar
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079659/
https://www.ncbi.nlm.nih.gov/pubmed/21443764
http://dx.doi.org/10.1186/1756-0500-4-84

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079659/
https://www.ncbi.nlm.nih.gov/pubmed/21443764
http://dx.doi.org/10.1186/1756-0500-4-84

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