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Artificial Modulation of the Gating Behavior of a K(+) Channel in a KvAP-DNA Chimera
We present experiments where the gating behavior of a voltage-gated ion channel is modulated by artificial ligand binding. We construct a channel-DNA chimera with the KvAP potassium channel reconstituted in an artificial membrane. The channel is functional and the single channel ion conductivity unp...
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079724/ https://www.ncbi.nlm.nih.gov/pubmed/21526187 http://dx.doi.org/10.1371/journal.pone.0018598 |
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author | Wang, Andrew Zocchi, Giovanni |
author_facet | Wang, Andrew Zocchi, Giovanni |
author_sort | Wang, Andrew |
collection | PubMed |
description | We present experiments where the gating behavior of a voltage-gated ion channel is modulated by artificial ligand binding. We construct a channel-DNA chimera with the KvAP potassium channel reconstituted in an artificial membrane. The channel is functional and the single channel ion conductivity unperturbed by the presence of the DNA. However, the channel opening probability vs. bias voltage, i.e., the gating, can be shifted considerably by the electrostatic force between the charges on the DNA and the voltage sensing domain of the protein. Different hybridization states of the chimera DNA thus lead to different response curves of the channel. |
format | Text |
id | pubmed-3079724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30797242011-04-27 Artificial Modulation of the Gating Behavior of a K(+) Channel in a KvAP-DNA Chimera Wang, Andrew Zocchi, Giovanni PLoS One Research Article We present experiments where the gating behavior of a voltage-gated ion channel is modulated by artificial ligand binding. We construct a channel-DNA chimera with the KvAP potassium channel reconstituted in an artificial membrane. The channel is functional and the single channel ion conductivity unperturbed by the presence of the DNA. However, the channel opening probability vs. bias voltage, i.e., the gating, can be shifted considerably by the electrostatic force between the charges on the DNA and the voltage sensing domain of the protein. Different hybridization states of the chimera DNA thus lead to different response curves of the channel. Public Library of Science 2011-04-19 /pmc/articles/PMC3079724/ /pubmed/21526187 http://dx.doi.org/10.1371/journal.pone.0018598 Text en Wang, Zocchi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Andrew Zocchi, Giovanni Artificial Modulation of the Gating Behavior of a K(+) Channel in a KvAP-DNA Chimera |
title | Artificial Modulation of the Gating Behavior of a K(+) Channel in a KvAP-DNA Chimera |
title_full | Artificial Modulation of the Gating Behavior of a K(+) Channel in a KvAP-DNA Chimera |
title_fullStr | Artificial Modulation of the Gating Behavior of a K(+) Channel in a KvAP-DNA Chimera |
title_full_unstemmed | Artificial Modulation of the Gating Behavior of a K(+) Channel in a KvAP-DNA Chimera |
title_short | Artificial Modulation of the Gating Behavior of a K(+) Channel in a KvAP-DNA Chimera |
title_sort | artificial modulation of the gating behavior of a k(+) channel in a kvap-dna chimera |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3079724/ https://www.ncbi.nlm.nih.gov/pubmed/21526187 http://dx.doi.org/10.1371/journal.pone.0018598 |
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