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Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium

Differentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type–specific features. Understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles, which have been challenging in primary tissue samples due to...

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Autores principales: Maruyama, Reo, Choudhury, Sibgat, Kowalczyk, Adam, Bessarabova, Marina, Beresford-Smith, Bryan, Conway, Thomas, Kaspi, Antony, Wu, Zhenhua, Nikolskaya, Tatiana, Merino, Vanessa F., Lo, Pang-Kuo, Liu, X. Shirley, Nikolsky, Yuri, Sukumar, Saraswati, Haviv, Izhak, Polyak, Kornelia
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080862/
https://www.ncbi.nlm.nih.gov/pubmed/21533021
http://dx.doi.org/10.1371/journal.pgen.1001369
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author Maruyama, Reo
Choudhury, Sibgat
Kowalczyk, Adam
Bessarabova, Marina
Beresford-Smith, Bryan
Conway, Thomas
Kaspi, Antony
Wu, Zhenhua
Nikolskaya, Tatiana
Merino, Vanessa F.
Lo, Pang-Kuo
Liu, X. Shirley
Nikolsky, Yuri
Sukumar, Saraswati
Haviv, Izhak
Polyak, Kornelia
author_facet Maruyama, Reo
Choudhury, Sibgat
Kowalczyk, Adam
Bessarabova, Marina
Beresford-Smith, Bryan
Conway, Thomas
Kaspi, Antony
Wu, Zhenhua
Nikolskaya, Tatiana
Merino, Vanessa F.
Lo, Pang-Kuo
Liu, X. Shirley
Nikolsky, Yuri
Sukumar, Saraswati
Haviv, Izhak
Polyak, Kornelia
author_sort Maruyama, Reo
collection PubMed
description Differentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type–specific features. Understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles, which have been challenging in primary tissue samples due to limited numbers of cells available. Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, as well as gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type–specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation.
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spelling pubmed-30808622011-04-29 Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium Maruyama, Reo Choudhury, Sibgat Kowalczyk, Adam Bessarabova, Marina Beresford-Smith, Bryan Conway, Thomas Kaspi, Antony Wu, Zhenhua Nikolskaya, Tatiana Merino, Vanessa F. Lo, Pang-Kuo Liu, X. Shirley Nikolsky, Yuri Sukumar, Saraswati Haviv, Izhak Polyak, Kornelia PLoS Genet Research Article Differentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type–specific features. Understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles, which have been challenging in primary tissue samples due to limited numbers of cells available. Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, as well as gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type–specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation. Public Library of Science 2011-04-21 /pmc/articles/PMC3080862/ /pubmed/21533021 http://dx.doi.org/10.1371/journal.pgen.1001369 Text en Maruyama et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Maruyama, Reo
Choudhury, Sibgat
Kowalczyk, Adam
Bessarabova, Marina
Beresford-Smith, Bryan
Conway, Thomas
Kaspi, Antony
Wu, Zhenhua
Nikolskaya, Tatiana
Merino, Vanessa F.
Lo, Pang-Kuo
Liu, X. Shirley
Nikolsky, Yuri
Sukumar, Saraswati
Haviv, Izhak
Polyak, Kornelia
Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title_full Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title_fullStr Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title_full_unstemmed Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title_short Epigenetic Regulation of Cell Type–Specific Expression Patterns in the Human Mammary Epithelium
title_sort epigenetic regulation of cell type–specific expression patterns in the human mammary epithelium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080862/
https://www.ncbi.nlm.nih.gov/pubmed/21533021
http://dx.doi.org/10.1371/journal.pgen.1001369
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