Functional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA

The Leu294 residue in the cytoplasmic neck of Thermotoga maritima CorA is considered to be the main gate for Mg(2+) transport. We created three site-directed mutants at this position: in the Leu294Asp and Leu294Gly mutants we observed a defect in closing of the pore, while in the Leu294Arg mutant no...

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Detalles Bibliográficos
Autores principales: Svidová, Soňa, Sponder, Gerhard, Schweyen, Rudolf J., Djinović-Carugo, Kristina
Formato: Texto
Lenguaje:English
Publicado: Elsevier Pub. Co 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082049/
https://www.ncbi.nlm.nih.gov/pubmed/21074514
http://dx.doi.org/10.1016/j.bbamem.2010.10.017
Descripción
Sumario:The Leu294 residue in the cytoplasmic neck of Thermotoga maritima CorA is considered to be the main gate for Mg(2+) transport. We created three site-directed mutants at this position: in the Leu294Asp and Leu294Gly mutants we observed a defect in closing of the pore, while in the Leu294Arg mutant not only gating, but also the regulation of Mg(2+) uptake was affected. Our results confirmed the importance of the Leu294 for gating of Mg(2+) transport and in addition revealed the influence of the charge and structural features of the amino acid residues on the gating mechanism.

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