Functional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA

The Leu294 residue in the cytoplasmic neck of Thermotoga maritima CorA is considered to be the main gate for Mg(2+) transport. We created three site-directed mutants at this position: in the Leu294Asp and Leu294Gly mutants we observed a defect in closing of the pore, while in the Leu294Arg mutant no...

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Autores principales: Svidová, Soňa, Sponder, Gerhard, Schweyen, Rudolf J., Djinović-Carugo, Kristina
Formato: Texto
Lenguaje:English
Publicado: Elsevier Pub. Co 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082049/
https://www.ncbi.nlm.nih.gov/pubmed/21074514
http://dx.doi.org/10.1016/j.bbamem.2010.10.017
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author Svidová, Soňa
Sponder, Gerhard
Schweyen, Rudolf J.
Djinović-Carugo, Kristina
author_facet Svidová, Soňa
Sponder, Gerhard
Schweyen, Rudolf J.
Djinović-Carugo, Kristina
author_sort Svidová, Soňa
collection PubMed
description The Leu294 residue in the cytoplasmic neck of Thermotoga maritima CorA is considered to be the main gate for Mg(2+) transport. We created three site-directed mutants at this position: in the Leu294Asp and Leu294Gly mutants we observed a defect in closing of the pore, while in the Leu294Arg mutant not only gating, but also the regulation of Mg(2+) uptake was affected. Our results confirmed the importance of the Leu294 for gating of Mg(2+) transport and in addition revealed the influence of the charge and structural features of the amino acid residues on the gating mechanism.
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spelling pubmed-30820492011-06-01 Functional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA Svidová, Soňa Sponder, Gerhard Schweyen, Rudolf J. Djinović-Carugo, Kristina Biochim Biophys Acta Article The Leu294 residue in the cytoplasmic neck of Thermotoga maritima CorA is considered to be the main gate for Mg(2+) transport. We created three site-directed mutants at this position: in the Leu294Asp and Leu294Gly mutants we observed a defect in closing of the pore, while in the Leu294Arg mutant not only gating, but also the regulation of Mg(2+) uptake was affected. Our results confirmed the importance of the Leu294 for gating of Mg(2+) transport and in addition revealed the influence of the charge and structural features of the amino acid residues on the gating mechanism. Elsevier Pub. Co 2011-06 /pmc/articles/PMC3082049/ /pubmed/21074514 http://dx.doi.org/10.1016/j.bbamem.2010.10.017 Text en © 2011 Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
spellingShingle Article
Svidová, Soňa
Sponder, Gerhard
Schweyen, Rudolf J.
Djinović-Carugo, Kristina
Functional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA
title Functional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA
title_full Functional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA
title_fullStr Functional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA
title_full_unstemmed Functional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA
title_short Functional analysis of the conserved hydrophobic gate region of the magnesium transporter CorA
title_sort functional analysis of the conserved hydrophobic gate region of the magnesium transporter cora
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082049/
https://www.ncbi.nlm.nih.gov/pubmed/21074514
http://dx.doi.org/10.1016/j.bbamem.2010.10.017
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