High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection

Protein expression in heterologous hosts for functional studies is a cumbersome effort. Here, we report a superior platform for parallel protein expression in vivo and in vitro. The platform combines highly efficient ligation-independent cloning (LIC) with instantaneous detection of expressed protei...

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Detalles Bibliográficos
Autores principales: Dortay, Hakan, Akula, Usha Madhuri, Westphal, Christin, Sittig, Marie, Mueller-Roeber, Bernd
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082538/
https://www.ncbi.nlm.nih.gov/pubmed/21541323
http://dx.doi.org/10.1371/journal.pone.0018900

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082538/
https://www.ncbi.nlm.nih.gov/pubmed/21541323
http://dx.doi.org/10.1371/journal.pone.0018900

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