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Hepatitis E virus ORF2 protein over-expressed by baculovirus in hepatoma cells, efficiently encapsidates and transmits the viral RNA to naïve cells

A recombinant baculovirus(vBacORF2) that expressed the full-length ORF2 capsid protein of a genotype 1 strain of hepatitis E virus(HEV) was constructed. Transduction of S10-3 human hepatoma cells with this baculovirus led to large amounts of ORF2 protein production in ~50% of the cells as determined...

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Detalles Bibliográficos
Autores principales: Parvez, Mohammad K, Purcell, Robert H, Emerson, Suzanne U
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3083364/
https://www.ncbi.nlm.nih.gov/pubmed/21477278
http://dx.doi.org/10.1186/1743-422X-8-159