Programmable Sequence-Specific Transcriptional Regulation of Mammalian Genome Using Designer TAL Effectors

The ability to direct functional domains to specific DNA sequences is a long sought-after goal for studying and engineering biological processes. Transcription activator like effectors (TALEs) from Xanthomonas sp. present a promising platform for designing sequence-specific DNA binding proteins. Her...

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Detalles Bibliográficos
Autores principales: Zhang, Feng, Cong, Le, Lodato, Simona, Kosuri, Sriram, Church, George, Arlotta, Paola
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084533/
https://www.ncbi.nlm.nih.gov/pubmed/21248753
http://dx.doi.org/10.1038/nbt.1775
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author Zhang, Feng
Cong, Le
Lodato, Simona
Kosuri, Sriram
Church, George
Arlotta, Paola
author_facet Zhang, Feng
Cong, Le
Lodato, Simona
Kosuri, Sriram
Church, George
Arlotta, Paola
author_sort Zhang, Feng
collection PubMed
description The ability to direct functional domains to specific DNA sequences is a long sought-after goal for studying and engineering biological processes. Transcription activator like effectors (TALEs) from Xanthomonas sp. present a promising platform for designing sequence-specific DNA binding proteins. Here we describe a robust and rapid method for overcoming the difficulty of constructing TALE repeat domains. We synthesized 17 designer TALEs (dTALEs) that are customized to recognize specific DNA binding sites, and demonstrate that dTALEs can specifically modulate transcription of endogenous genes (Sox2 and Klf4) from the native genome in human cells. dTALEs provide a designable DNA targeting platform for the interrogation and engineering of biological systems.
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spelling pubmed-30845332011-08-01 Programmable Sequence-Specific Transcriptional Regulation of Mammalian Genome Using Designer TAL Effectors Zhang, Feng Cong, Le Lodato, Simona Kosuri, Sriram Church, George Arlotta, Paola Nat Biotechnol Article The ability to direct functional domains to specific DNA sequences is a long sought-after goal for studying and engineering biological processes. Transcription activator like effectors (TALEs) from Xanthomonas sp. present a promising platform for designing sequence-specific DNA binding proteins. Here we describe a robust and rapid method for overcoming the difficulty of constructing TALE repeat domains. We synthesized 17 designer TALEs (dTALEs) that are customized to recognize specific DNA binding sites, and demonstrate that dTALEs can specifically modulate transcription of endogenous genes (Sox2 and Klf4) from the native genome in human cells. dTALEs provide a designable DNA targeting platform for the interrogation and engineering of biological systems. 2011-01-19 2011-02 /pmc/articles/PMC3084533/ /pubmed/21248753 http://dx.doi.org/10.1038/nbt.1775 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Zhang, Feng
Cong, Le
Lodato, Simona
Kosuri, Sriram
Church, George
Arlotta, Paola
Programmable Sequence-Specific Transcriptional Regulation of Mammalian Genome Using Designer TAL Effectors
title Programmable Sequence-Specific Transcriptional Regulation of Mammalian Genome Using Designer TAL Effectors
title_full Programmable Sequence-Specific Transcriptional Regulation of Mammalian Genome Using Designer TAL Effectors
title_fullStr Programmable Sequence-Specific Transcriptional Regulation of Mammalian Genome Using Designer TAL Effectors
title_full_unstemmed Programmable Sequence-Specific Transcriptional Regulation of Mammalian Genome Using Designer TAL Effectors
title_short Programmable Sequence-Specific Transcriptional Regulation of Mammalian Genome Using Designer TAL Effectors
title_sort programmable sequence-specific transcriptional regulation of mammalian genome using designer tal effectors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084533/
https://www.ncbi.nlm.nih.gov/pubmed/21248753
http://dx.doi.org/10.1038/nbt.1775
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