A mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range

FRET-based sensors for cyclic Adenosine Mono Phosphate (cAMP) have revolutionized the way in which this important intracellular messenger is studied. The currently prevailing sensors consist of the cAMP-binding protein Epac1, sandwiched between suitable donor- and acceptor fluorescent proteins (FPs)...

Descripción completa

Detalles Bibliográficos
Autores principales: Klarenbeek, Jeffrey B., Goedhart, Joachim, Hink, Mark A., Gadella, Theodorus W. J., Jalink, Kees
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084777/
https://www.ncbi.nlm.nih.gov/pubmed/21559477
http://dx.doi.org/10.1371/journal.pone.0019170

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084777/
https://www.ncbi.nlm.nih.gov/pubmed/21559477
http://dx.doi.org/10.1371/journal.pone.0019170

Ejemplares similares