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A mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range

FRET-based sensors for cyclic Adenosine Mono Phosphate (cAMP) have revolutionized the way in which this important intracellular messenger is studied. The currently prevailing sensors consist of the cAMP-binding protein Epac1, sandwiched between suitable donor- and acceptor fluorescent proteins (FPs)...

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Detalles Bibliográficos
Autores principales:	Klarenbeek, Jeffrey B., Goedhart, Joachim, Hink, Mark A., Gadella, Theodorus W. J., Jalink, Kees
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3084777/ https://www.ncbi.nlm.nih.gov/pubmed/21559477 http://dx.doi.org/10.1371/journal.pone.0019170

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