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M153R Mutation in a pH-Sensitive Green Fluorescent Protein Stabilizes Its Fusion Proteins
BACKGROUND: Green fluorescent protein (GFP) and its fusion proteins have been used extensively to monitor and analyze a wide range of biological processes. However, proteolytic cleavage often removes GFP from its fusion proteins, not only causing a poor signal-to-noise ratio of the fluorescent image...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086926/ https://www.ncbi.nlm.nih.gov/pubmed/21559297 http://dx.doi.org/10.1371/journal.pone.0019598 |