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Reliability analysis of the Ahringer Caenorhabditis elegans RNAi feeding library: a guide for genome-wide screens

BACKGROUND: The Ahringer C. elegans RNAi feeding library prepared by cloning genomic DNA fragments has been widely used in genome-wide analysis of gene function. However, the library has not been thoroughly validated by direct sequencing, and there are potential errors, including: 1) mis-annotation...

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Detalles Bibliográficos
Autores principales: Qu, Wubin, Ren, Changhong, Li, Yuan, Shi, Jinping, Zhang, Jiye, Wang, Xiaolei, Hang, Xingyi, Lu, Yiming, Zhao, Dongsheng, Zhang, Chenggang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087708/
https://www.ncbi.nlm.nih.gov/pubmed/21453524
http://dx.doi.org/10.1186/1471-2164-12-170