Cargando…

Reliability analysis of the Ahringer Caenorhabditis elegans RNAi feeding library: a guide for genome-wide screens

BACKGROUND: The Ahringer C. elegans RNAi feeding library prepared by cloning genomic DNA fragments has been widely used in genome-wide analysis of gene function. However, the library has not been thoroughly validated by direct sequencing, and there are potential errors, including: 1) mis-annotation...

Descripción completa

Detalles Bibliográficos
Autores principales:	Qu, Wubin, Ren, Changhong, Li, Yuan, Shi, Jinping, Zhang, Jiye, Wang, Xiaolei, Hang, Xingyi, Lu, Yiming, Zhao, Dongsheng, Zhang, Chenggang
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087708/ https://www.ncbi.nlm.nih.gov/pubmed/21453524 http://dx.doi.org/10.1186/1471-2164-12-170

Ejemplares similares

A proteomic view of Caenorhabditis elegans caused by short-term hypoxic stress
por: Li, Hualing, et al.
Publicado: (2010)

MPprimer: a program for reliable multiplex PCR primer design
por: Shen, Zhiyong, et al.
Publicado: (2010)

Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans
por: Yan, Qitao, et al.
Publicado: (2009)

The Effectiveness of RNAi in Caenorhabditis elegans Is Maintained during Spaceflight
por: Etheridge, Timothy, et al.
Publicado: (2011)

Genome-Wide RNAi Longevity Screens in Caenorhabditis elegans
por: Yanos, Melana E, et al.
Publicado: (2012)

Natural and Unanticipated Modifiers of RNAi Activity in Caenorhabditis elegans
por: Asad, Nadeem, et al.
Publicado: (2012)

Chemically defined medium and Caenorhabditis elegans
por: Szewczyk, Nathaniel J, et al.
Publicado: (2003)

High-throughput reverse genetics: RNAi screens in Caenorhabditis elegans
por: Bargmann, Cornelia I
Publicado: (2001)

New Strains for Tissue-Specific RNAi Studies in Caenorhabditis elegans
por: Watts, Jason S., et al.
Publicado: (2020)

TILLING is an effective reverse genetics technique for Caenorhabditis elegans
por: Gilchrist, Erin J, et al.
Publicado: (2006)

A novel in vitro Caenorhabditis elegans transcription system
por: Wibisono, Phillip, et al.
Publicado: (2020)

Novel insights into RNAi off-target effects using C. elegans paralogs
por: Rual, Jean-François, et al.
Publicado: (2007)

Inhibiting miRNA in Caenorhabditis elegans using a potent and selective antisense reagent
por: Zheng, Genhua, et al.
Publicado: (2010)

Genome-wide modeling of complex phenotypes in Caenorhabditis elegans and Drosophila melanogaster
por: De, Supriyo, et al.
Publicado: (2013)

Overcoming Redundancy: An RNAi Enhancer Screen for Morphogenesis Genes in Caenorhabditis elegans
por: Sawyer, Jacob M., et al.
Publicado: (2011)

Clone Mapper: An Online Suite of Tools for RNAi Experiments in Caenorhabditis elegans
por: Thakur, Nishant, et al.
Publicado: (2014)

Visualization and Quantification of Transposon Activity in Caenorhabditis elegans RNAi Pathway Mutants
por: Wallis, Dylan C., et al.
Publicado: (2019)

An endomembrane zinc transporter negatively regulates systemic RNAi in Caenorhabditis elegans
por: Dejima, Katsufumi, et al.
Publicado: (2023)

Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi
por: Yoshida, Keita, et al.
Publicado: (2023)

A fitness assay for comparing RNAi effects across multiple C. elegans genotypes
por: Elvin, Mark, et al.
Publicado: (2011)

RNAi targeting Caenorhabditis elegans α-arrestins has little effect on lifespan
por: Park, Sangsoon, et al.
Publicado: (2017)

Circular Estimate Method (CEM) - a Simple Method to Estimate Caenorhabditis elegans Culture Densities in Liquid Medium
por: Josende, Marcelo Estrella, et al.
Publicado: (2019)

A Genome-Wide RNAi Screen for Factors Involved in Neuronal Specification in Caenorhabditis elegans
por: Poole, Richard J., et al.
Publicado: (2011)

An RNAi-Based Suppressor Screen Identifies Interactors of the Myt1 Ortholog of Caenorhabditis elegans
por: Allen, Anna K., et al.
Publicado: (2014)

RNAi Screen Identifies Novel Regulators of RNP Granules in the Caenorhabditis elegans Germ Line
por: Wood, Megan P., et al.
Publicado: (2016)

Selection of Reliable Reference Genes in Caenorhabditis elegans for Analysis of Nanotoxicity
por: Zhang, Yanqiong, et al.
Publicado: (2012)

MFEprimer-2.0: a fast thermodynamics-based program for checking PCR primer specificity
por: Qu, Wubin, et al.
Publicado: (2012)

Use of SNPs to determine the breakpoints of complex deficiencies, facilitating gene mapping in Caenorhabditis elegans
por: Kadandale, Pavan, et al.
Publicado: (2005)

Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method
por: Kage-Nakadai, Eriko, et al.
Publicado: (2012)

An economical and highly adaptable optogenetics system for individual and population-level manipulation of Caenorhabditis elegans
por: Koopman, M., et al.
Publicado: (2021)

A genome-wide RNAi screen identifies factors required for distinct stages of C. elegans piRNA biogenesis
por: Goh, Wee-Siong Sho, et al.
Publicado: (2014)

Curcumin supplementation increases longevity and antioxidant capacity in Caenorhabditis elegans
por: Xu, Jianing, et al.
Publicado: (2023)

A Conserved PHD Finger Protein and Endogenous RNAi Modulate Insulin Signaling in Caenorhabditis elegans
por: Mansisidor, Andres R., et al.
Publicado: (2011)

The miR-35-41 Family of MicroRNAs Regulates RNAi Sensitivity in Caenorhabditis elegans
por: Massirer, Katlin B., et al.
Publicado: (2012)

Identification of Genes Interacting with rnt-1 Through Large-Scale RNAi Screening in Caenorhabditis elegans
por: Lee, Kiho, et al.
Publicado: (2013)

Caenorhabditis elegans germ granules are present in distinct configurations that differentially associate with RNAi-targeted RNAs
por: Uebel, Celja J., et al.
Publicado: (2023)

Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans
por: Hernando-Rodríguez, Blanca, et al.
Publicado: (2018)

Nymphal RNAi: systemic RNAi mediated gene knockdown in juvenile grasshopper
por: Dong, Ying, et al.
Publicado: (2005)

Hydrogen extends Caenorhabditis elegans longevity by reducing reactive oxygen species
por: Zhang, Miao, et al.
Publicado: (2020)

Risperidone induced alterations in feeding and locomotion behavior of Caenorhabditis elegans
por: Gaur, Aaditya Vikram, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_fd8qsft2r3motkm8kcrdcq2r6u