Degenerate sequence recognition by the monomeric restriction enzyme: single mutation converts BcnI into a strand-specific nicking endonuclease

Unlike orthodox Type II restriction endonucleases that are homodimers and interact with the palindromic 4–8-bp DNA sequences, BcnI is a monomer which has a single active site but cuts both DNA strands within the 5′-CC↓CGG-3′/3′-GGG↓CC-5′ target site (‘↓’ designates the cleavage position). Therefore,...

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Detalles Bibliográficos
Autores principales: Kostiuk, Georgij, Sasnauskas, Giedrius, Tamulaitiene, Giedre, Siksnys, Virginijus
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089477/
https://www.ncbi.nlm.nih.gov/pubmed/21227928
http://dx.doi.org/10.1093/nar/gkq1351

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089477/
https://www.ncbi.nlm.nih.gov/pubmed/21227928
http://dx.doi.org/10.1093/nar/gkq1351

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