Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2′-deoxyuridine

We report procedures to allow incorporation and detection of 5-ethynyl-2′-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromodeoxyuridine. Low concentrations of EdU (1 µM) are sufficient to allow detection of incorporation in cells expressi...

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Detalles Bibliográficos
Autores principales: Hua, Hui, Kearsey, Stephen E.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089489/
https://www.ncbi.nlm.nih.gov/pubmed/21310713
http://dx.doi.org/10.1093/nar/gkr063

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089489/
https://www.ncbi.nlm.nih.gov/pubmed/21310713
http://dx.doi.org/10.1093/nar/gkr063

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