Quantitative Cell-based Protein Degradation Assays to Identify and Classify Drugs That Target the Ubiquitin-Proteasome System

We have generated a set of dual-reporter human cell lines and devised a chase protocol to quantify proteasomal degradation of a ubiquitin fusion degradation (UFD) substrate, a ubiquitin ligase CRL2(VHL) substrate, and a ubiquitin-independent substrate. Well characterized inhibitors that target diffe...

Descripción completa

Detalles Bibliográficos
Autores principales: Chou, Tsui-Fen, Deshaies, Raymond J.
Formato: Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2011
Materias:
Protein Synthesis and Degradation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089497/
https://www.ncbi.nlm.nih.gov/pubmed/21343295
http://dx.doi.org/10.1074/jbc.M110.215319

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089497/
https://www.ncbi.nlm.nih.gov/pubmed/21343295
http://dx.doi.org/10.1074/jbc.M110.215319

Ejemplares similares