Cargando…

Quantitative Cell-based Protein Degradation Assays to Identify and Classify Drugs That Target the Ubiquitin-Proteasome System

We have generated a set of dual-reporter human cell lines and devised a chase protocol to quantify proteasomal degradation of a ubiquitin fusion degradation (UFD) substrate, a ubiquitin ligase CRL2(VHL) substrate, and a ubiquitin-independent substrate. Well characterized inhibitors that target diffe...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chou, Tsui-Fen, Deshaies, Raymond J.
Formato:	Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2011
Materias:	Protein Synthesis and Degradation
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3089497/ https://www.ncbi.nlm.nih.gov/pubmed/21343295 http://dx.doi.org/10.1074/jbc.M110.215319

Ejemplares similares

Multiple Sclerosis Autoantigen Myelin Basic Protein Escapes Control by Ubiquitination during Proteasomal Degradation
por: Belogurov, Alexey, et al.
Publicado: (2014)

Atypical Ubiquitylation in Yeast Targets Lysine-less Asi2 for Proteasomal Degradation
por: Boban, Mirta, et al.
Publicado: (2015)

CRL3(IBTK) Regulates the Tumor Suppressor Pdcd4 through Ubiquitylation Coupled to Proteasomal Degradation
por: Pisano, Antonio, et al.
Publicado: (2015)

Isopeptide and ester bond ubiquitination both regulate degradation of the human dopamine receptor 4
por: Peeler, Jennifer C., et al.
Publicado: (2017)

The Archaeal Proteasome Is Regulated by a Network of AAA ATPases
por: Forouzan, Dara, et al.
Publicado: (2012)

Conserved Sequence Preferences Contribute to Substrate Recognition by the Proteasome
por: Yu, Houqing, et al.
Publicado: (2016)

Membrane-associated Ubiquitin Ligase Complex Containing gp78 Mediates Sterol-accelerated Degradation of 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase
por: Jo, Youngah, et al.
Publicado: (2011)

Starvation Induces Proteasome Autophagy with Different Pathways for Core and Regulatory Particles
por: Waite, Kenrick A., et al.
Publicado: (2016)

Structural insights into non-covalent ubiquitin activation of the cIAP1-UbcH5B∼ubiquitin complex
por: Patel, Amrita, et al.
Publicado: (2019)

Preferential digestion of PCNA-ubiquitin and p53-ubiquitin linkages by USP7 to remove polyubiquitin chains from substrates
por: Masuda, Yuji, et al.
Publicado: (2019)

Structural characterization of the bacterial proteasome homolog BPH reveals a tetradecameric double-ring complex with unique inner cavity properties
por: Fuchs, Adrian C. D., et al.
Publicado: (2018)

Ubiquitination of the N-terminal Region of Caveolin-1 Regulates Endosomal Sorting by the VCP/p97 AAA-ATPase
por: Kirchner, Philipp, et al.
Publicado: (2013)

The deubiquitinase USP7 uses a distinct ubiquitin-like domain to deubiquitinate NF-ĸB subunits
por: Mitxitorena, Izaskun, et al.
Publicado: (2020)

Biochemical and Structural Studies of a HECT-like Ubiquitin Ligase from Escherichia coli O157:H7
por: Lin, David Yin-wei, et al.
Publicado: (2011)

A suite of mathematical solutions to describe ternary complex formation and their application to targeted protein degradation by heterobifunctional ligands
por: Han, Bomie
Publicado: (2020)

The endosomal trafficking regulator LITAF controls the cardiac Nav1.5 channel via the ubiquitin ligase NEDD4-2
por: Turan, Nilüfer N., et al.
Publicado: (2021)

OTUB1 non-catalytically stabilizes the E2 ubiquitin-conjugating enzyme UBE2E1 by preventing its autoubiquitination
por: Pasupala, Nagesh, et al.
Publicado: (2018)

E3 Ligase SCF(βTrCP)-induced DYRK1A Protein Degradation Is Essential for Cell Cycle Progression in HEK293 Cells
por: Liu, Qiang, et al.
Publicado: (2016)

The Acidic Tail of the Cdc34 Ubiquitin-conjugating Enzyme Functions in Both Binding to and Catalysis with Ubiquitin Ligase SCF(Cdc4)
por: Kleiger, Gary, et al.
Publicado: (2009)

Yeast 18 S rRNA Is Directly Involved in the Ribosomal Response to Stringent AUG Selection during Translation Initiation
por: Nemoto, Naoki, et al.
Publicado: (2010)

Self-assembly of Severe Acute Respiratory Syndrome Coronavirus Membrane Protein
por: Tseng, Ying-Tzu, et al.
Publicado: (2010)

Sterol-induced Dislocation of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase from Endoplasmic Reticulum Membranes into the Cytosol through a Subcellular Compartment Resembling Lipid Droplets
por: Hartman, Isamu Z., et al.
Publicado: (2010)

Repair of Isoaspartate Formation Modulates the Interaction of Deamidated 4E-BP2 with mTORC1 in Brain
por: Bidinosti, Michael, et al.
Publicado: (2010)

Further in Vitro Exploration Fails to Support the Allosteric Three-site Model
por: Petropoulos, Alexandros D., et al.
Publicado: (2012)

Identification of Intersubunit Domain Interactions within Eukaryotic Initiation Factor (eIF) 2B, the Nucleotide Exchange Factor for Translation Initiation
por: Reid, Peter J., et al.
Publicado: (2012)

Control of Activating Transcription Factor 4 (ATF4) Persistence by Multisite Phosphorylation Impacts Cell Cycle Progression and Neurogenesis
por: Frank, Christopher L., et al.
Publicado: (2010)

Functional Characterization of the Role of the N-terminal Domain of the c/Nip1 Subunit of Eukaryotic Initiation Factor 3 (eIF3) in AUG Recognition
por: Karásková, Martina, et al.
Publicado: (2012)

The Reduction Potential of the Active Site Disulfides of Human Protein Disulfide Isomerase Limits Oxidation of the Enzyme by Ero1α
por: Chambers, Joseph E., et al.
Publicado: (2010)

New Hierarchical Phosphorylation Pathway of the Translational Repressor eIF4E-binding Protein 1 (4E-BP1) in Ischemia-Reperfusion Stress
por: Ayuso, María I., et al.
Publicado: (2010)

Norovirus Translation Requires an Interaction between the C Terminus of the Genome-linked Viral Protein VPg and Eukaryotic Translation Initiation Factor 4G
por: Chung, Liliane, et al.
Publicado: (2014)

In Vitro Assembly of Catalase
por: Baureder, Michael, et al.
Publicado: (2014)

The Recognition of Collagen and Triple-helical Toolkit Peptides by MMP-13: SEQUENCE SPECIFICITY FOR BINDING AND CLEAVAGE
por: Howes, Joanna-Marie, et al.
Publicado: (2014)

Eukaryotic Release Factor 3 Is Required for Multiple Turnovers of Peptide Release Catalysis by Eukaryotic Release Factor 1
por: Eyler, Daniel E., et al.
Publicado: (2013)

A Nascent Peptide Signal Responsive to Endogenous Levels of Polyamines Acts to Stimulate Regulatory Frameshifting on Antizyme mRNA
por: Yordanova, Martina M., et al.
Publicado: (2015)

A Translation System Reconstituted with Human Factors Proves That Processing of Encephalomyocarditis Virus Proteins 2A and 2B Occurs in the Elongation Phase of Translation without Eukaryotic Release Factors
por: Machida, Kodai, et al.
Publicado: (2014)

Naturally Occurring Isoleucyl-tRNA Synthetase without tRNA-dependent Pre-transfer Editing
por: Cvetesic, Nevena, et al.
Publicado: (2016)

The Ribosome Restrains Molten Globule Formation in Stalled Nascent Flavodoxin
por: Houwman, Joseline A., et al.
Publicado: (2016)

Three human aminoacyl-tRNA synthetases have distinct sub-mitochondrial localizations that are unaffected by disease-associated mutations
por: González-Serrano, Ligia Elena, et al.
Publicado: (2018)

A broad-spectrum antiviral molecule, QL47, selectively inhibits eukaryotic translation
por: de Wispelaere, Mélissanne, et al.
Publicado: (2020)

Stop codon read-through of mammalian MTCH2 leading to an unstable isoform regulates mitochondrial membrane potential
por: Manjunath, Lekha E., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_dfkildn1okmmv3al3qj470nprm