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Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system

BACKGROUND: Recently, some groups have reported on cell-free synthesis of functional membrane proteins (MPs) in the presence of exogenous liposomes (liposomes). Previously, we reported synthesis of a functional AtPPT1 plant phosphate transporter that was associated with liposomes during translation....

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Detalles Bibliográficos
Autores principales: Nozawa, Akira, Ogasawara, Tomio, Matsunaga, Satoko, Iwasaki, Takahiro, Sawasaki, Tatsuya, Endo, Yaeta
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090341/
https://www.ncbi.nlm.nih.gov/pubmed/21481249
http://dx.doi.org/10.1186/1472-6750-11-35