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Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood
Enrichment of rare circulating tumor cells (CTCs) in blood is typically achieved using antibodies to epithelial cell adhesion molecule (EpCAM), with detection using cytokeratin (CK) antibodies. However, EpCAM and CK are not expressed in some tumors and can be downregulated during epithelial-to-mesen...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090615/ https://www.ncbi.nlm.nih.gov/pubmed/21577258 http://dx.doi.org/10.1155/2011/252361 |
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author | Mikolajczyk, Stephen D. Millar, Lisa S. Tsinberg, Pavel Coutts, Stephen M. Zomorrodi, Maryam Pham, Tam Bischoff, Farideh Z. Pircher, Tony J. |
author_facet | Mikolajczyk, Stephen D. Millar, Lisa S. Tsinberg, Pavel Coutts, Stephen M. Zomorrodi, Maryam Pham, Tam Bischoff, Farideh Z. Pircher, Tony J. |
author_sort | Mikolajczyk, Stephen D. |
collection | PubMed |
description | Enrichment of rare circulating tumor cells (CTCs) in blood is typically achieved using antibodies to epithelial cell adhesion molecule (EpCAM), with detection using cytokeratin (CK) antibodies. However, EpCAM and CK are not expressed in some tumors and can be downregulated during epithelial-to-mesenchymal transition. A micro-fluidic system, not limited to EpCAM or CK, was developed to use multiple antibodies for capture followed by detection using CEE-Enhanced (CE), a novel in situ staining method that fluorescently labels the capture antibodies bound to CTCs. Higher recovery of CTCs was demonstrated using antibody mixtures compared to anti-EpCAM. In addition, CK-positive breast cancer cells were found in 15 of 24 samples (63%; range 1–60 CTCs), while all samples contained additional CE-positive cells (range 1–41; median = 11; P = .02). Thus, antibody mixtures against a range of cell surface antigens enables capture of more CTCs than anti-EpCAM alone and CE staining enables the detection of CK-negative CTCs. |
format | Text |
id | pubmed-3090615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-30906152011-05-16 Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood Mikolajczyk, Stephen D. Millar, Lisa S. Tsinberg, Pavel Coutts, Stephen M. Zomorrodi, Maryam Pham, Tam Bischoff, Farideh Z. Pircher, Tony J. J Oncol Research Article Enrichment of rare circulating tumor cells (CTCs) in blood is typically achieved using antibodies to epithelial cell adhesion molecule (EpCAM), with detection using cytokeratin (CK) antibodies. However, EpCAM and CK are not expressed in some tumors and can be downregulated during epithelial-to-mesenchymal transition. A micro-fluidic system, not limited to EpCAM or CK, was developed to use multiple antibodies for capture followed by detection using CEE-Enhanced (CE), a novel in situ staining method that fluorescently labels the capture antibodies bound to CTCs. Higher recovery of CTCs was demonstrated using antibody mixtures compared to anti-EpCAM. In addition, CK-positive breast cancer cells were found in 15 of 24 samples (63%; range 1–60 CTCs), while all samples contained additional CE-positive cells (range 1–41; median = 11; P = .02). Thus, antibody mixtures against a range of cell surface antigens enables capture of more CTCs than anti-EpCAM alone and CE staining enables the detection of CK-negative CTCs. Hindawi Publishing Corporation 2011 2011-04-19 /pmc/articles/PMC3090615/ /pubmed/21577258 http://dx.doi.org/10.1155/2011/252361 Text en Copyright © 2011 Stephen D. Mikolajczyk et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mikolajczyk, Stephen D. Millar, Lisa S. Tsinberg, Pavel Coutts, Stephen M. Zomorrodi, Maryam Pham, Tam Bischoff, Farideh Z. Pircher, Tony J. Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood |
title | Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood |
title_full | Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood |
title_fullStr | Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood |
title_full_unstemmed | Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood |
title_short | Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood |
title_sort | detection of epcam-negative and cytokeratin-negative circulating tumor cells in peripheral blood |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090615/ https://www.ncbi.nlm.nih.gov/pubmed/21577258 http://dx.doi.org/10.1155/2011/252361 |
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